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IGBP1 Polyclonal antibody

IGBP1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14952-1-AP

Synonyms

ALPHA 4, CD79a binding protein 1, IBP1, IGBP1, Protein alpha 4

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using IGBP1 Polyclonal antibody (14952-1-AP)

    WB analysis of Jurkat using 14952-1-AP

    WB result of IGBP1 antibody (14952-1-AP, 1:1000) with si-control and si-IGBP1 transfected Jurkat cells.

  • Western Blot (WB) analysis of Raji cells using IGBP1 Polyclonal antibody (14952-1-AP)

    WB analysis of Raji using 14952-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using IGBP1 Polyclonal antibody (14952-1-AP)

    WB analysis of Jurkat using 14952-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using IGBP1 Polyclonal antibody (14952-1-AP)

    WB analysis of HeLa using 14952-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using IGBP1 Polyclonal antibody (14952-1-AP)

    WB analysis of MCF-7 using 14952-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using IGBP1 Polyclonal antibody (14952-1-AP)

    IP experiment of Jurkat using 14952-1-AP

    IP result of anti-IGBP1 (IP:14952-1-AP, 4ug; Detection:14952-1-AP 1:300) with Jurkat cells lysate 4000 ug.

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Tested Applications

Positive WB detected inRaji cells, Jurkat
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14952-1-AP targets IGBP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGBP1 fusion protein Ag6782 相同性解析による交差性が予測される生物種
Full Name immunoglobulin (CD79A) binding protein 1
Calculated molecular weight 39 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC004137
Gene symbol IGBP1
Gene ID (NCBI) 3476
RRIDAB_2121990
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Immunoglobulin-binding protein 1 (IGBP1), also known as ALPHA-4, was originally identified as a signal transduction molecule coprecipitating with MB1 (CD79a) protein of the B-cell antigen receptor (BCR) complex. IGBP1 has been characterized as an essential regulator of the catalytic activity of protein phosphatase 2A (PP2A), which is a ubiquitous phosphatase with broad substrate specificity and diverse cellular functions.

Protocols

Product Specific Protocols
WB protocol for IGBP1 antibody 14952-1-APDownload protocol
IP protocol for IGBP1 antibody 14952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols