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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using IGBP1 Polyclonal antibody (14952-1-AP)

    WB analysis of Jurkat using 14952-1-AP

    WB result of IGBP1 antibody (14952-1-AP, 1:1000) with si-control and si-IGBP1 transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using IGBP1 Polyclonal antibody (14952-1-AP)

    WB analysis using 14952-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using IGBP1 Polyclonal antibody (14952-1-AP)

    IP experiment of Jurkat using 14952-1-AP

    IP result of anti-IGBP1 (IP:14952-1-AP, 4ug; Detection:14952-1-AP 1:300) with Jurkat cells lysate 4000 ug.

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  • KD/KO Validated

IGBP1 Polyclonal antibody

IGBP1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 14952-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Immunoglobulin-binding protein 1, IBP1, CD79a-binding protein 1, CD79a binding protein 1, B-cell signal transduction molecule alpha 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells, Raji cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14952-1-AP targets IGBP1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGBP1 fusion protein Ag6782 相同性解析による交差性が予測される生物種
Full Name immunoglobulin (CD79A) binding protein 1
Calculated molecular weight 39 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC004137
Gene Symbol IGBP1
Gene ID (NCBI) 3476
RRIDAB_2121990
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Immunoglobulin-binding protein 1 (IGBP1), also known as ALPHA-4, was originally identified as a signal transduction molecule coprecipitating with MB1 (CD79a) protein of the B-cell antigen receptor (BCR) complex. IGBP1 has been characterized as an essential regulator of the catalytic activity of protein phosphatase 2A (PP2A), which is a ubiquitous phosphatase with broad substrate specificity and diverse cellular functions.

Protocols

Product Specific Protocols
WB protocol for IGBP1 antibody 14952-1-APDownload protocol
IP protocol for IGBP1 antibody 14952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols