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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGBP1 Monoclonal antibody

IGBP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D11A8

Cat no : 60256-1-Ig

Synonyms

ALPHA 4, CD79a binding protein 1, IBP1, IGBP1, Protein alpha 4

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using IGBP1 Monoclonal antibody (60256-1-Ig)

    WB analysis of K-562 using 60256-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 60256-1-Ig (IGBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using IGBP1 Monoclonal antibody (60256-1-Ig)

    IHC staining of human heart using 60256-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60256-1-Ig (IGBP1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using IGBP1 Monoclonal antibody (60256-1-Ig)

    IHC staining of human heart using 60256-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60256-1-Ig (IGBP1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using IGBP1 Monoclonal antibody (60256-1-Ig)

    IHC staining of human skeletal muscle using 60256-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60256-1-Ig (IGBP1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using IGBP1 Monoclonal antibody (60256-1-Ig)

    IHC staining of human skeletal muscle using 60256-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60256-1-Ig (IGBP1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 60256-1-Ig is IGBP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IGBP1 fusion protein Ag6782 相同性解析による交差性が予測される生物種
Full Name immunoglobulin (CD79A) binding protein 1
Calculated molecular weight 39 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC004137
Gene symbol IGBP1
Gene ID (NCBI) 3476
RRIDAB_2881377
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Immunoglobulin-binding protein 1 (IGBP1), also known as ALPHA-4, was originally identified as a signal transduction molecule coprecipitating with MB1 (CD79a) protein of the B-cell antigen receptor (BCR) complex. IGBP1 has been characterized as an essential regulator of the catalytic activity of protein phosphatase 2A (PP2A), which is a ubiquitous phosphatase with broad substrate specificity and diverse cellular functions.

Protocols

Product Specific Protocols
WB protocol for IGBP1 antibody 60256-1-IgDownload protocol
IHC protocol for IGBP1 antibody 60256-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols