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  • KD/KO Validated

IGF1B-Specific Polyclonal antibody

IGF1B-Specific Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

IHC, IF-P, Dot blot, ELISA

Conjugate

Unconjugated

Cat no : 20215-1-AP

Synonyms

IGF-1, IGF1, Insulin like growth factor I, IGF-I, IGFI


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Tested Applications

Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20215-1-AP targets IGF1B-Specific in IHC, IF-P, Dot blot, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor 1 (somatomedin C)
Calculated molecular weight 22 kDa
GenBank accession numberNM_001111285
Gene symbol IGF1
Gene ID (NCBI) 3479
RRIDAB_10697837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF1B, also named as IBP1, MGF, IGF-I and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but have a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. The antibody is specific to isoform IGF-1B.

Protocols

Product Specific Protocols
IHC protocol for IGF1B-Specific antibody 20215-1-APDownload protocol
IF protocol for IGF1B-Specific antibody 20215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseDot blot

Environ Health Perspect

Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.

Authors - Jacob Raber
chicken

Oxid Med Cell Longev

IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart.

Authors - Yafan Gong
rat

Cell Death Dis

miR-129 controls axonal regeneration via regulating insulin-like growth factor-1 in peripheral nerve injury.

Authors - Hui Zhu
  • KD Validated
human

Front Bioeng Biotechnol

Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.

Authors - Kaile Zhang

Chem Biol Interact

Oenothein B induced human non-small cell lung cancer A549 cell death by ROS-mediated PI3K/Akt/NF-κB signaling pathway.

Authors - Xiaodong Pei

Mol Cell Biol

Histone Citrullination Represses MicroRNA Expression, Resulting in Increased Oncogene mRNAs in Somatolactotrope Cells.

Authors - Stanley B DeVore