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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGF1R Monoclonal antibody (66283-1-Ig)

    WB analysis using 66283-1-Ig (same clone as 66283-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using IGF1R Monoclonal antibody (66283-1-Ig)

    WB analysis of HUVEC using 66283-1-Ig (same clone as 66283-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using IGF1R Monoclonal antibody (66283-1-Ig)

    WB analysis of HepG2 using 66283-1-Ig (same clone as 66283-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using IGF1R Monoclonal antibody (66283-1-Ig)

    WB analysis of HeLa using 66283-1-Ig (same clone as 66283-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using IGF1R Monoclonal antibody (66283-1-Ig)

    WB analysis of MCF-7 using 66283-1-Ig (same clone as 66283-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IGF1R Monoclonal antibody (66283-1-Ig)

    IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IGF1R Monoclonal antibody (66283-1-Ig)

    IHC staining of human breast cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using IGF1R Monoclonal antibody (66283-1-Ig)

    IHC staining of human endometrial cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using IGF1R Monoclonal antibody (66283-1-Ig)

    IHC staining of human endometrial cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IGF1R Monoclonal antibody (66283-1-Ig)

    IHC staining of human lung cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IGF1R Monoclonal antibody (66283-1-Ig)

    IHC staining of human lung cancer using 66283-1-Ig (same clone as 66283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66283-1-PBS in a different storage buffer formulation.

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IGF1R Monoclonal antibody, PBS Only

IGF1R Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66283-1-PBS
Clone No.3C6A11

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

IGF 1 receptor, IGF-I receptor, IGFIR, IGFR, IGF I receptor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66283-1-PBS targets IGF1R in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name IGF I receptor
Calculated molecular weight 155 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberNM_000875
Gene Symbol IGF1R
Gene ID (NCBI) 3480
RRIDAB_2881666
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08069
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is a heterotetrameric receptor, composed of two alpha subunits (130 kDa each) and two beta subunits (~98 kDa each) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.