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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGF1R Recombinant antibody (84385-3-RR)

    WB analysis using 84385-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84385-3-RR (IGF1R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84385-3-RR

    Affinity and Kinetic Characterization of 84385-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84385-3-RR against Human IGF1R were performed. The affinity constant is 0.239 nM.

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IGF1R Recombinant antibody

IGF1R Recombinant Antibody for WB, ELISA
Cat No. 84385-3-RR
Clone No.241678B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IGF 1 receptor, IGF-I receptor, IGFIR, IGFR, IGF I receptor

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, A549 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84385-3-RR targets IGF1R in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name IGF I receptor
Calculated molecular weight 1367 aa, 155 kDa
Observed molecular weight130 kDa
GenBank accession numberBC113612
Gene Symbol IGF1R
Gene ID (NCBI) 3480
RRIDAB_3671921
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08069
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is a heterotetrameric receptor, composed of two alpha subunits (130 kDa each) and two beta subunits (~98 kDa each) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.

Protocols

Product Specific Protocols
WB protocol for IGF1R antibody 84385-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols