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IGF2BP1 Polyclonal antibody

Name: IGF2BP1 Polyclonal antibody
Brand: Proteintech
SKU: 22803-1-AP
Rating: 4.67 (3 reviews)

IGF2BP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (3)

Cat no : 22803-1-AP

Synonyms

IMP 1, IGF-II mRNA-binding protein 1, IGF2BP 1, IGF2 mRNA-binding protein 1, CRD-BP

Validation Data Gallery

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AllWBIPIHCIF/ICC

WB analysis of Jurkat using 22803-1-AP
WB result of IGF2BP1 antibody (22803-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IGF2BP1 transfected Jurkat cells.
WB analysis of Jurkat using 22803-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 22803-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22803-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 22803-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 22803-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 22803-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HuH-7 using 22803-1-AP
HuH-7 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 22803-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22803-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22803-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22803-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 22803-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 22803-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 22803-1-AP
IP result of anti-IGF2BP1 (IP:22803-1-AP, 4ug; Detection:22803-1-AP 1:1000) with HEK-293 cells lysate 1480ug.
IHC staining of human lung cancer using 22803-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 22803-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 22803-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 22803-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A375 using 22803-1-AP
Immunofluorescent analysis of A375 cells using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of A375 using 22803-1-AP
Immunofluorescent analysis of (4% PFA) fixed A375 cells using IGF2BP1 antibody (22803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Cat No. 22803-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells, mouse kidney tissue, A375 cells, rat kidney tissue, HepG2 cells, HuH-7 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human lung cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A375 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 21 publications below
WB	See 68 publications below
IHC	See 11 publications below
IF	See 9 publications below
IP	See 2 publications below
CoIP	See 3 publications below
RIP	See 16 publications below

Product Information

22803-1-AP targets IGF2BP1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IGF2BP1 fusion protein Ag18859 相同性解析による交差性が予測される生物種
Full Name	insulin-like growth factor 2 mRNA binding protein 1
Calculated molecular weight	577 aa, 63 kDa
Observed molecular weight	65-70 kDa
GenBank accession number	BC156957
Gene symbol	IGF2BP1
Gene ID (NCBI)	10642
RRID	AB_2879173
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IGF2BP1, also named as CRDBP, VICKZ1, ZBP1, is a 577 amino acid protein, which belongs to the RRM IMP/VICKZ family. IGF2BP1 can form homodimers and heterodimers with IGF2BP1 and IGF2BP3. IGF2BP1 is mainly expressed in the embryo, including in fetal liver, fetal lung, fetal kidney, fetal thymus and also expressed follicles of ovary, as well as in gonocytes of testis, spermatogonia, semen, oocytes and placenta. IGF2BP1 is Expressed in various cancers, including testis and lung cancers, as well as kidney, prostate and trachea cancers. IGF2BP1 as RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. The calcualted molecular weight of IGF2BP1 is 63 kDa, but modified IGF2BP1 is about 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for IGF2BP1 antibody 22803-1-AP	Download protocol
IHC protocol for IGF2BP1 antibody 22803-1-AP	Download protocol
IF protocol for IGF2BP1 antibody 22803-1-AP	Download protocol
IP protocol for IGF2BP1 antibody 22803-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs. Authors - Lei Sun KD Validated View Article
human	WB	Cell An in vitro system to silence mitochondrial gene expression. Authors - Luis Daniel Cruz-Zaragoza KD Validated View Article
human	WB	Cell Res Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells. Authors - Fei Peng View Article
mouse	IHC,IF,WB	ACS Cent Sci Allosteric Regulation of IGF2BP1 as a Novel Strategy for the Activation of Tumor Immune Microenvironment Authors - Yang Liu View Article
human	WB	Mol Cell m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3 Authors - Ting Shan KD Validated View Article
human	WB	Nat Commun The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma. Authors - Zhe Li View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Roy (Verified Customer) (06-13-2024)	Worked very well to detect IGF2BP1 at around 70 kDa by WB in HeLa cells (with an overnight incubation at 4°C, 1/1000 dilution) Applications: Western Blot Primary Antibody Dilution: 1/1000 Cell Tissue Type: HeLa cells
FH tao (Verified Customer) (12-14-2022)	Immunofluorescence seems to work, but signal can be detected in the nucleus Applications: Immunofluorescence Primary Antibody Dilution: 1:50
FH Parisa (Verified Customer) (06-13-2022)	I used the antibody and it worked very well, It was sensitive and could detect the faint band that I expected in my IP. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Hek293

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGF2BP1 Polyclonal antibody

IGF2BP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of Jurkat using 22803-1-AP

WB analysis of Jurkat using 22803-1-AP

WB analysis using 22803-1-AP

WB analysis of HEK-293 using 22803-1-AP

WB analysis of A375 using 22803-1-AP

WB analysis of rat kidney using 22803-1-AP

WB analysis of HepG2 using 22803-1-AP

WB analysis of HuH-7 using 22803-1-AP

WB analysis of Jurkat using 22803-1-AP

WB analysis of HEK-293 using 22803-1-AP

WB analysis of HEK-293 using 22803-1-AP

WB analysis of HEK-293 using 22803-1-AP

WB analysis of Jurkat using 22803-1-AP

WB analysis of mouse kidney using 22803-1-AP

IP experiment of HEK-293 using 22803-1-AP

IHC staining of human lung cancer using 22803-1-AP

IHC staining of human lung cancer using 22803-1-AP

IHC staining of human liver cancer using 22803-1-AP

IHC staining of human liver cancer using 22803-1-AP

IF Staining of A375 using 22803-1-AP

IF Staining of A375 using 22803-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

An in vitro system to silence mitochondrial gene expression.

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Allosteric Regulation of IGF2BP1 as a Novel Strategy for the Activation of Tumor Immune Microenvironment

m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3

The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.

Reviews