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  • KD/KO Validated

IGF2BP2 Polyclonal antibody

IGF2BP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 11601-1-AP

Synonyms

IGF II mRNA binding protein 2, IGF2 mRNA binding protein 2, IGF2BP2, IMP 2, IMP2, p62, VICKZ family member 2, VICKZ2


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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue, human kidney tissue, human heart tissue, COLO 320 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue, human colon cancer tissue, mouse stomach tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11601-1-AP targets IGF2BP2 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGF2BP2 fusion protein Ag2142 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor 2 mRNA binding protein 2
Calculated molecular weight 594 aa, 65 kDa
Observed molecular weight55-65 kDa
GenBank accession numberBC021290
Gene symbol IGF2BP2
Gene ID (NCBI) 10644
RRIDAB_2122672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2), ia a member of a family of mRNA binding protein, which can bind to several mammalian cells' mRNAs. Thus it may involve in the regulation of translation of mRNA. IGF2BP2's polymorphisms is risk factor for developing type 2 diabetes. This antibody raise against part of N-terminus of human IGF2BP2. IGF2BP2 has some isoforms with the MW of 54-66 kDa. Some literature has reported that multiple bands can be detected (PMID: 22427968, 36322753, 23069990).

Protocols

Product Specific Protocols
WB protocol for IGF2BP2 antibody 11601-1-APDownload protocol
IHC protocol for IGF2BP2 antibody 11601-1-APDownload protocol
IP protocol for IGF2BP2 antibody 11601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

An in vitro system to silence mitochondrial gene expression.

Authors - Luis Daniel Cruz-Zaragoza
humanIF

Mol Cancer

m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma

Authors - Han Fang
mouseWB

Cell Mol Immunol

Inhibition of METTL3 in macrophages provides protection against intestinal inflammation

Authors - Huilong Yin
humanWB,CoIP,RIP

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng
  • KD Validated
mouseRIP

Nat Cell Biol

N6-methyladenosine regulates maternal RNA maintenance in oocytes and timely RNA decay during mouse maternal-to-zygotic transition.

Authors - You Wu
humanWB,IF

Mol Cell

m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3

Authors - Ting Shan
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Parisa (Verified Customer) (06-13-2022)

good quality and specify.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293