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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGF2BP2 Monoclonal antibody

IGF2BP2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E5H8

Cat no : 60647-1-Ig

Synonyms

insulin like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, IGF-II mRNA-binding protein 2, IGF2 mRNA-binding protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGF2BP2 Monoclonal antibody (60647-1-Ig)

    WB analysis using 60647-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60647-1-Ig (IGF2BP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IGF2BP2 Monoclonal antibody (60647-1-Ig)

    WB analysis using 60647-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60647-1-Ig (IGF2BP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHepG2 cells, HT-1080 cells, A431 cells, HuH-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60647-1-Ig targets IGF2BP2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IGF2BP2 fusion protein Ag33681 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor 2 mRNA binding protein 2
Calculated molecular weight65 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC021290
Gene symbol IGF2BP2
Gene ID (NCBI) 10644
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2), ia a member of a family of mRNA binding protein, which can bind to several mammalian cells' mRNAs. Thus it may involve in the regulation of translation of mRNA. IGF2BP2's polymorphisms is risk factor for developing type 2 diabetes. This antibody raise against part of N-terminus of human IGF2BP2. IGF2BP2 has some isoforms with the MW of 54-66 kDa. Some literature has reported that multiple bands can be detected (PMID: 22427968, 36322753, 23069990).

Protocols

Product Specific Protocols
WB protocol for IGF2BP2 antibody 60647-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols