Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 14642-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14642-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14642-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 14642-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14642-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14642-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 14642-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14642-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 14642-1-AP
IP result of anti-IGF2BP3 (IP:14642-1-AP, 5ug; Detection:14642-1-AP 1:2000) with HEK-293 cells lysate 1040 ug.
IHC staining of human pancreas using 14642-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14642-1-AP (IGF2BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 14642-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14642-1-AP (IGF2BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 14642-1-AP
Immunofluorescent analysis of MCF-7 cells, using IGF2BP3 antibody 14642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Featured Product
KD/KO Validated

IGF2BP3 Polyclonal antibody

IGF2BP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14642-1-AP

Publications(131)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

IMP-3, IMP3, IMP 3, IGF-II mRNA-binding protein 3, IGF2BP 3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (131)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse placenta tissue, BxPC-3 cells, NIH/3T3 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 43 publications below
WB	See 106 publications below
IHC	See 31 publications below
IF	See 17 publications below
IP	See 13 publications below
CoIP	See 3 publications below
RIP	See 34 publications below

Product Information

14642-1-AP targets IGF2BP3 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IGF2BP3 fusion protein Ag6226 相同性解析による交差性が予測される生物種
Full Name	insulin-like growth factor 2 mRNA binding protein 3
Calculated molecular weight	64 kDa
Observed molecular weight	64 kDa
GenBank accession number	BC065269
Gene Symbol	IGF2BP3
Gene ID (NCBI)	10643
RRID	AB_2122782
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00425
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

Protocols

Product Specific Protocols
WB protocol for IGF2BP3 antibody 14642-1-AP	Download protocol
IHC protocol for IGF2BP3 antibody 14642-1-AP	Download protocol
IF protocol for IGF2BP3 antibody 14642-1-AP	Download protocol
IP protocol for IGF2BP3 antibody 14642-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell SIRT6 Suppresses Pancreatic Cancer through Control of Lin28b. Authors - Sita Kugel View Article
mouse	WB	Cancer Cell Lin28b is sufficient to drive liver cancer and necessary for its maintenance in murine models. Authors - Liem H Nguyen KD Validated View Article
mouse	WB	Cell Mol Immunol Inhibition of METTL3 in macrophages provides protection against intestinal inflammation Authors - Huilong Yin View Article
human	WB	Cell Res Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells. Authors - Fei Peng View Article
mouse	RIP	Nat Cell Biol N6-methyladenosine regulates maternal RNA maintenance in oocytes and timely RNA decay during mouse maternal-to-zygotic transition. Authors - You Wu View Article
human	WB	Mol Cell m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3 Authors - Ting Shan KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14642-1-AP

WB analysis of HeLa using 14642-1-AP

WB analysis of HeLa using 14642-1-AP

WB analysis of BxPC-3 using 14642-1-AP

WB analysis of HeLa using 14642-1-AP

WB analysis of HepG2 using 14642-1-AP

WB analysis of mouse placenta using 14642-1-AP

WB analysis of mouse brain using 14642-1-AP

IP experiment of HEK-293 using 14642-1-AP

IHC staining of human pancreas using 14642-1-AP

IHC staining of human pancreas using 14642-1-AP

IF Staining of MCF-7 using 14642-1-AP

IGF2BP3 Polyclonal antibody

IGF2BP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SIRT6 Suppresses Pancreatic Cancer through Control of Lin28b.

Lin28b is sufficient to drive liver cancer and necessary for its maintenance in murine models.

Inhibition of METTL3 in macrophages provides protection against intestinal inflammation

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

N6-methyladenosine regulates maternal RNA maintenance in oocytes and timely RNA decay during mouse maternal-to-zygotic transition.

m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3