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IGF2BP3 Polyclonal antibody

Name: IGF2BP3 Polyclonal antibody
Brand: Proteintech
SKU: 14642-1-AP
Rating: 2.50 (2 reviews)

IGF2BP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

119

Reviews (2)

Cat no : 14642-1-AP

Synonyms

IMP-3, IMP3, IMP 3, IGF-II mRNA-binding protein 3, IGF2BP 3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 14642-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 14642-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14642-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14642-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14642-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 14642-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14642-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14642-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14642-1-AP (IGF2BP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 14642-1-AP
IP result of anti-IGF2BP3 (IP:14642-1-AP, 5ug; Detection:14642-1-AP 1:2000) with HEK-293 cells lysate 1040 ug.
IHC staining of human pancreas using 14642-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14642-1-AP (IGF2BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 14642-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14642-1-AP (IGF2BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 14642-1-AP
Immunofluorescent analysis of MCF-7 cells, using IGF2BP3 antibody 14642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Cat No. 14642-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse placenta tissue, BxPC-3 cells, NIH/3T3 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 40 publications below
WB	See 97 publications below
IHC	See 30 publications below
IF	See 16 publications below
IP	See 11 publications below
CoIP	See 3 publications below
RIP	See 26 publications below

Product Information

14642-1-AP targets IGF2BP3 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IGF2BP3 fusion protein Ag6226 相同性解析による交差性が予測される生物種
Full Name	insulin-like growth factor 2 mRNA binding protein 3
Calculated molecular weight	64 kDa
Observed molecular weight	64 kDa
GenBank accession number	BC065269
Gene symbol	IGF2BP3
Gene ID (NCBI)	10643
RRID	AB_2122782
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

Protocols

Product Specific Protocols
WB protocol for IGF2BP3 antibody 14642-1-AP	Download protocol
IHC protocol for IGF2BP3 antibody 14642-1-AP	Download protocol
IF protocol for IGF2BP3 antibody 14642-1-AP	Download protocol
IP protocol for IGF2BP3 antibody 14642-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell SIRT6 Suppresses Pancreatic Cancer through Control of Lin28b. Authors - Sita Kugel View Article
mouse	WB	Cancer Cell Lin28b is sufficient to drive liver cancer and necessary for its maintenance in murine models. Authors - Liem H Nguyen KD Validated View Article
mouse	WB	Cell Mol Immunol Inhibition of METTL3 in macrophages provides protection against intestinal inflammation Authors - Huilong Yin View Article
human	WB	Cell Res Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells. Authors - Fei Peng View Article
mouse	RIP	Nat Cell Biol N6-methyladenosine regulates maternal RNA maintenance in oocytes and timely RNA decay during mouse maternal-to-zygotic transition. Authors - You Wu View Article
human	WB	Mol Cell m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3 Authors - Ting Shan KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH honglei (Verified Customer) (01-17-2023)	This antibody can be used for immunofluorescence and mainly localizes in the nucleus Applications: Immunofluorescence Primary Antibody Dilution: 1:50 Cell Tissue Type: cell line
FH Jessica (Verified Customer) (10-29-2018)	Also binds exogenous expressed IGF2BP1-GFP protein. I also get a double band around 70 kDa that is likely IGF2BP1 and IGF2BP3. This antibody is not specific. Applications: Western Blot, Primary Antibody Dilution: 1:1500 Cell Tissue Type: Neuroblastoma cells (human)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGF2BP3 Polyclonal antibody

IGF2BP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 14642-1-AP

WB analysis of BxPC-3 using 14642-1-AP

WB analysis of HeLa using 14642-1-AP

WB analysis of HeLa using 14642-1-AP

WB analysis of mouse brain using 14642-1-AP

WB analysis of mouse placenta using 14642-1-AP

WB analysis of HeLa using 14642-1-AP

WB analysis of HepG2 using 14642-1-AP

IP experiment of HEK-293 using 14642-1-AP

IHC staining of human pancreas using 14642-1-AP

IHC staining of human pancreas using 14642-1-AP

IF Staining of MCF-7 using 14642-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SIRT6 Suppresses Pancreatic Cancer through Control of Lin28b.

Lin28b is sufficient to drive liver cancer and necessary for its maintenance in murine models.

Inhibition of METTL3 in macrophages provides protection against intestinal inflammation

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

N6-methyladenosine regulates maternal RNA maintenance in oocytes and timely RNA decay during mouse maternal-to-zygotic transition.

m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3

Reviews

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