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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGFBP1 Polyclonal antibody

IGFBP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31335-1-AP

Synonyms

IGF binding protein 1, IBP-1, IBP1, IBP 1, hIGFBP 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGFBP1 Polyclonal antibody (31335-1-AP)

    WB analysis using 31335-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 31335-1-AP (IGFBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31335-1-AP targets IGFBP1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name IGF binding protein 1
Calculated molecular weight28 kDa
Observed molecular weight28-35 kDa
GenBank accession numberNM_000596.4
Gene symbol IGFBP1
Gene ID (NCBI) 3484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF-binding proteins (IGFBPs) family contains members like IGFBP-1, -2, -3, -4,-5 and-6. IGFBPs prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGF-I physically interacts with IGFBP-1 and that IGFBP-1 also binds to an integrin receptor, but the effect of IGFBP-1 on cell migration may be independent of IGF-I and is probably mediated through the alpha 5 beta 1 integrin. Transgenic mice models demonstrated that IGFBPs played important roles in the pathogenesis of obesity and INS resistance. Studies conducted in humans demonstrated the close relation between IGFBPs and the components of the metabolic syndrome.

Protocols

Product Specific Protocols
WB protocol for IGFBP1 antibody 31335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols