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- KD/KO Validated
IGFBP2 Polyclonal antibody
IGFBP2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11065-3-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | SH-SY5Y cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 14 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
11065-3-AP targets IGFBP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, chicken, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IGFBP2 fusion protein Ag1535 相同性解析による交差性が予測される生物種 |
| Full Name | IGF binding protein 2 |
| Calculated molecular weight | 35 kDa |
| Observed molecular weight | 33-36 kDa |
| GenBank accession number | BC009902 |
| Gene symbol | IGFBP2 |
| Gene ID (NCBI) | 3485 |
| RRID | AB_2123101 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
INS-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kD IGFBP protein with degraded fragments of 24 kD, 19kd and 15kd. The levels of IGFBP2 are elevated during progression of many human cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IGFBP2 antibody 11065-3-AP | Download protocol |
| IHC protocol for IGFBP2 antibody 11065-3-AP | Download protocol |
| IP protocol for IGFBP2 antibody 11065-3-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.
| ||
Nat Commun Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism. | ||
Oxid Med Cell Longev IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart. | ||
Acta Physiol (Oxf) SARM1 promotes the neuroinflammation and demyelination through IGFBP2/NF-κB pathway in experimental autoimmune encephalomyelitis mice | ||
Front Immunol The cuproptosis-related signature associated with the tumor environment and prognosis of patients with glioma | ||
