Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 17 publications below |
| IHC | See 5 publications below |
| IF | See 2 publications below |
Product Information
11065-3-AP targets IGFBP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, chicken, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1535 Product name: Recombinant human IGFBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC009902 Sequence: MLPRVGCPALPLPPPPLLPLLPLLLLLLGASGGGGGARAEVLFRCPPCTPERLAACGPPPVAPPAAVAAVAGGARMPCAELVREPGCGCCSVCARLEGEACGVYTPRCGQGLRCYPHPGSELPLQALVMGEGTCEKRRDAEYGASPEQVADNGDDHSEGGLVENHVDSTMNMLGGGGSAGRKPLKSGMKELAVFREKVTEQHRQMGKGGKHHLGLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEARGVHTQRMQ 相同性解析による交差性が予測される生物種 |
| Full Name | IGF binding protein 2 |
| Calculated molecular weight | 35 kDa |
| Observed molecular weight | 33-36 kDa |
| GenBank accession number | BC009902 |
| Gene Symbol | IGFBP2 |
| Gene ID (NCBI) | 3485 |
| RRID | AB_2123101 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18065 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IGFBP2, also known as BP2 and IBP2, is a member of the IGFBP family. It is predominantly expressed in fetal tissues that are highly proliferative, such as the early post-implantation epiblast, the ventricular zone of the rostral neuroepithelium, and has been found to be highly expressed in many cancers (PMID: 31925333). It is a promising protein in non-communicable diseases such as obesity, insulin resistance, metabolic syndrome or type 2 diabetes (PMID: 33498859).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IGFBP2 antibody 11065-3-AP | Download protocol |
| IHC protocol for IGFBP2 antibody 11065-3-AP | Download protocol |
| IP protocol for IGFBP2 antibody 11065-3-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.
| ||
Nat Commun Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism. | ||
Oxid Med Cell Longev IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart. | ||
Acta Physiol (Oxf) SARM1 promotes the neuroinflammation and demyelination through IGFBP2/NF-κB pathway in experimental autoimmune encephalomyelitis mice | ||
Front Immunol The cuproptosis-related signature associated with the tumor environment and prognosis of patients with glioma |