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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGFBP2 Polyclonal antibody

IGFBP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15699-1-AP

Synonyms

IGF binding protein 2, IBP-2, IBP2, IBP 2, BP2

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using IGFBP2 Polyclonal antibody (15699-1-AP)

    WB analysis of MCF-7 using 15699-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 15699-1-AP (IGFBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IGFBP2 Polyclonal antibody (15699-1-AP)

    IHC staining of human pancreas cancer using 15699-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15699-1-AP (IGFBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IGFBP2 Polyclonal antibody (15699-1-AP)

    IHC staining of human pancreas cancer using 15699-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15699-1-AP (IGFBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IGFBP2 Polyclonal antibody (15699-1-AP)

    IF Staining of HeLa using 15699-1-AP

    Immunofluorescent analysis of HeLa cells using 15699-1-AP (IGFBP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15699-1-AP targets IGFBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGFBP2 fusion protein Ag8327 相同性解析による交差性が予測される生物種
Full Name IGF binding protein 2
Calculated molecular weight 36 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC004312
Gene symbol IGFBP2
Gene ID (NCBI) 3485
RRIDAB_10641053
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kD IGFBP protein with degarded fragments of 24 kD, 19kd and 15kd. The levels of IGFBP2 are elevated during progression of many human cancers. IGFBP2 is expressed in invasive PAc, whereas its expression in HG-PIN is low. It can be as an Immunohistochemical Marker for Prostatic Adenocarcinoma.

Protocols

Product Specific Protocols
WB protocol for IGFBP2 antibody 15699-1-APDownload protocol
IHC protocol for IGFBP2 antibody 15699-1-APDownload protocol
IF protocol for IGFBP2 antibody 15699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols