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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGFBP2 Polyclonal antibody

IGFBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31248-1-AP

Synonyms

IGF binding protein 2, IBP-2, IBP2, IBP 2, BP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGFBP2 Polyclonal antibody (31248-1-AP)

    WB analysis using 31248-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31248-1-AP (IGFBP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using IGFBP2 Polyclonal antibody (31248-1-AP)

    IHC staining of human ovary cancer using 31248-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31248-1-AP (IGFBP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293T cells, SH-SY5Y cells, human plasma
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31248-1-AP targets IGFBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name IGF binding protein 2
Calculated molecular weight35 kDa
Observed molecular weight35 kDa
GenBank accession numberNM_000597.3
Gene symbol IGFBP2
Gene ID (NCBI) 3485
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGFBP2, also known as BP2 and IBP2, is a member of the IGFBP family. It is predominantly expressed in fetal tissues that are highly proliferative, such as the early post-implantation epiblast, the ventricular zone of the rostral neuroepithelium, and has been found to be highly expressed in many cancers (PMID: 31925333). It is a promising protein in non-communicable diseases such as obesity, insulin resistance, metabolic syndrome or type 2 diabetes (PMID: 33498859).

Protocols

Product Specific Protocols
WB protocol for IGFBP2 antibody 31248-1-APDownload protocol
IHC protocol for IGFBP2 antibody 31248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols