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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGFBP2 Monoclonal antibody, PBS Only

IGFBP2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F10D1

Cat no : 66644-1-PBS

Synonyms

IBP-2, IBP2, IBP 2, BP2, BP 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGFBP2 Monoclonal antibody (66644-1-Ig)

    WB analysis using 66644-1-Ig (same clone as 66644-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66644-1-Ig (IGFBP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66644-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using IGFBP2 Monoclonal antibody (66644-1-Ig)

    IF Staining of A549 using 66644-1-Ig (same clone as 66644-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IGFBP2 antibody (66644-1-Ig, Clone: 2F10D1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66644-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66644-1-PBS targets IGFBP2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IGFBP2 fusion protein Ag8421 相同性解析による交差性が予測される生物種
Full Name IGF binding protein 2
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004312
Gene symbol IGFBP2
Gene ID (NCBI) 3485
RRIDAB_2882002
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. The levels of IGFBP2 are elevated during progression of many human cancers.