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  • KD/KO Validated

IGFBP3 Polyclonal antibody

IGFBP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10189-2-AP

Synonyms

IGF-binding protein 3, IGF binding protein 3, IBP-3, IBP3, IBP 3


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Tested Applications

Positive WB detected inmouse colon tissue, HeLa cells, A2780 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10189-2-AP targets IGFBP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGFBP3 fusion protein Ag0242 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor binding protein 3
Calculated molecular weight 32 kDa
Observed molecular weight 32-37 kDa
GenBank accession numberBC000013
Gene symbol IGFBP3
Gene ID (NCBI) 3486
RRIDAB_2123233
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)

Protocols

Product Specific Protocols
WB protocol for IGFBP3 antibody 10189-2-APDownload protocol
IHC protocol for IGFBP3 antibody 10189-2-APDownload protocol
IF protocol for IGFBP3 antibody 10189-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

3D reconstruction of a gastrulating human embryo

Authors - Zhenyu Xiao
human,mouseWB,IHC,IF

Theranostics

Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p

Authors - Soo Hyun Kang
  • KD Validated

Proc Natl Acad Sci U S A

Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.

Authors - Liang Chen
mouseWB

Acta Physiol (Oxf)

Podocyte apoptosis in diabetic nephropathy by BASP1 activation of the p53 pathway via WT1.

Authors - Yingying Zhang
humanWB

Cancer Lett

Choline-induced SLC5A7 impairs colorectal cancer growth by stabilizing p53 protein.

Authors - Yuan Yin
mouseWB

PLoS Biol

The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis.

Authors - Patrick A Carroll

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Margarita (Verified Customer) (04-19-2023)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: PC3 cells
IGFBP3 Antibody Immunofluorescence validation (1:100 dilution) in PC3 cells (Cat no:10189-2-AP)