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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGFBP3 Recombinant antibody, PBS Only (Detector)

IGFBP3 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241723A6

Cat no : 84470-1-PBS

Synonyms

IGF binding protein 3, IBP-3, IBP3, IBP 3, BP 53

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01352-2

    Sandwich ELISA standard curve of MP01352-2

    Sandwich ELISA standard curve of MP01352-2, Human IGFBP3 Recombinant Matched Antibody Pair - PBS only. 84470-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1827. 84470-1-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL.

  • Sample test of MP01352-2

    Sample test of MP01352-2

    A431 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human IGFBP3, and measured 44.22 ng/mL.

  • Sample test of MP01352-2

    Sample test of MP01352-2

    Serum of six individual healthy human donors was measured. The IGFBP3 concentration of detected samples was determined to be 9,505.05 ng/mL with a range of 7,790.22-10,751.16 ng/mL.

  • Cytometric bead array standard curve of MP01352-1

    Cytometric bead array standard curve of MP01352-1

    Cytometric bead array standard curve of MP01352-1, IGFBP3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84470-2-PBS. Detection antibody: 84470-1-PBS. Standard: Eg1827. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01352-2

    Cytometric bead array standard curve of MP01352-2

    Cytometric bead array standard curve of MP01352-2, IGFBP3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84470-3-PBS. Detection antibody: 84470-1-PBS. Standard: Eg1827. Range: 0.625-80 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84470-1-PBS targets IGFBP3 as part of a matched antibody pair:

MP01352-1: 84470-2-PBS capture and 84470-1-PBS detection (validated in Cytometric bead array)

MP01352-2: 84470-3-PBS capture and 84470-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor binding protein 3
Calculated molecular weight32 kDa
GenBank accession numberNM_000598.5
Gene symbol IGFBP3
Gene ID (NCBI) 3486
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.