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Validation Data Gallery

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  • Western Blot (WB) analysis of C6 cells using IGFBP4 Polyclonal antibody (18500-1-AP)

    WB analysis of C6 using 18500-1-AP (same clone as 18500-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 18500-1-AP (IGFBP4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using IGFBP4 Polyclonal antibody (18500-1-AP)

    WB analysis of C6 using 18500-1-AP (same clone as 18500-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 18500-1-AP (IGFBP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using IGFBP4 Polyclonal antibody (18500-1-AP)

    IHC staining of human liver using 18500-1-AP (same clone as 18500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using IGFBP4 Polyclonal antibody (18500-1-AP)

    IHC staining of human liver using 18500-1-AP (same clone as 18500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using IGFBP4 Polyclonal antibody (18500-1-AP)

    IHC staining of human colon using 18500-1-AP (same clone as 18500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using IGFBP4 Polyclonal antibody (18500-1-AP)

    IHC staining of human colon using 18500-1-AP (same clone as 18500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using IGFBP4 Polyclonal antibody (18500-1-AP)

    IF Staining of C6 using 18500-1-AP (same clone as 18500-1-PBS)

    Immunofluorescent analysis of C6 cells using 18500-1-AP (IGFBP4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18500-1-PBS targets IGFBP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGFBP4 fusion protein Ag13347 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor binding protein 4
Calculated molecular weight 28 kDa
Observed molecular weight 28-40 kDa
GenBank accession numberBC016041
Gene Symbol IGFBP4
Gene ID (NCBI) 3487
RRIDAB_2878547
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22692
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401). IGFBP4 is a secreted protein and calculated molecular weight is 28 kDa.