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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGFBP4 Recombinant antibody, PBS Only (Detector)

IGFBP4 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230543B11

Cat no : 83200-1-PBS

Synonyms

IBP 4, HT29 IGFBP, Deb2, BP4, BP 4

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00279-2

    Sandwich ELISA standard curve of MP00279-2

    Sandwich ELISA standard curve of MP00279-2, Human IGFBP4 Recombinant Matched Antibody Pair - PBS only. 83200-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0825. 83200-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP00279-2

    Sample test of MP00279-2

    A549 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The mean IGFBP4 concentration was determined to be 181.3 ng/mL in A549 cell supernatant.

  • Sample test of MP00279-2

    Sample test of MP00279-2

    Serum of sixteen individual healthy human donors was measured. The IGFBP4 concentration of detected samples was determined to be 36.9 ng/mL with a range of 4.7 - 129.3 ng/mL.

  • Cytometric bead array standard curve of MP00279-1

    Cytometric bead array standard curve of MP00279-1

    Cytometric bead array standard curve of MP00279-1, Human IGFBP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83200-2-PBS. Detection antibody: 83200-1-PBS. Standard: Eg0825. Range: 1.56-50 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83200-1-PBS targets IGFBP4 as part of a matched antibody pair:

MP00279-1: 83200-2-PBS capture and 83200-1-PBS detection (validated in Cytometric bead array)

MP00279-2: 83200-4-PBS capture and 83200-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor binding protein 4
Calculated molecular weight28 kDa
GenBank accession numberNM_001552.3
Gene symbol IGFBP4
Gene ID (NCBI) 3487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.