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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGFBP4 Recombinant antibody, PBS Only

IGFBP4 Recombinant Antibody for WB, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230543F8

Cat no : 83200-3-PBS

Synonyms

BP 4, HT29 IGFBP, IBP 4, IBP4, IGF binding protein 4, IGFBP 4, IGFBP4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGFBP4 Recombinant antibody (83200-3-RR)

    WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IGFBP4 Recombinant antibody (83200-3-RR)

    WB analysis using 83200-3-RR (same clone as 83200-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using IGFBP4 Recombinant antibody (83200-3-RR)

    FC experiment of A549 using 83200-3-RR (same clone as 83200-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.4 ug IGFBP4 Recombinant antibody (83200-3-RR, Clone:230543F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83200-3-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83200-3-PBS is IGFBP4 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor binding protein 4
Calculated molecular weight28 kDa
GenBank accession numberNM_001552.3
Gene symbol IGFBP4
Gene ID (NCBI) 3487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.