Validation Data Gallery
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Tested Applications
| Positive WB detected in | SKOV-3 cells, HepG2 cells, mouse embryo tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 14 publications below |
| IHC | See 3 publications below |
| IF | See 5 publications below |
Product Information
55205-1-AP targets IGFBP5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | insulin-like growth factor binding protein 5 |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | NM_000599 |
| Gene Symbol | IGFBP5 |
| Gene ID (NCBI) | 3488 |
| RRID | AB_2736835 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P24593 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IGFBP5 antibody 55205-1-AP | Download protocol |
| IHC protocol for IGFBP5 antibody 55205-1-AP | Download protocol |
| IF protocol for IGFBP5 antibody 55205-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Material-induced Senescence (MIS): Fluidity Induces Senescent Type Cell Death of Lung Cancer Cells via Insulin-Like Growth Factor Binding Protein 5. | ||
Breast Cancer Res Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors | ||
J Proteomics Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation. | ||
Am J Physiol Heart Circ Physiol Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5. |