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Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using IGFBP5 Polyclonal antibody (55205-1-AP)

    WB analysis of SKOV-3 using 55205-1-AP (same clone as 55205-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 55205-1-AP (IGFBP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55205-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IGFBP5 Polyclonal antibody (55205-1-AP)

    WB analysis using 55205-1-AP (same clone as 55205-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55205-1-AP (IGFBP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55205-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse embryo tissue using IGFBP5 Polyclonal antibody (55205-1-AP)

    WB analysis of mouse embryo using 55205-1-AP (same clone as 55205-1-PBS)

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 55205-1-AP (IGFBP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55205-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IGFBP5 Polyclonal antibody (55205-1-AP)

    IHC staining of human breast cancer using 55205-1-AP (same clone as 55205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55205-1-AP (IGFBP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55205-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IGFBP5 Polyclonal antibody (55205-1-AP)

    IHC staining of human breast cancer using 55205-1-AP (same clone as 55205-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55205-1-AP (IGFBP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55205-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using IGFBP5 Polyclonal antibody (55205-1-AP)

    IF Staining of mouse brain using 55205-1-AP (same clone as 55205-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using IGFBP5 antibody (55205-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55205-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55205-1-PBS targets IGFBP5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor binding protein 5
Calculated molecular weight 31 kDa
Observed molecular weight 33 kDa
GenBank accession numberNM_000599
Gene Symbol IGFBP5
Gene ID (NCBI) 3488
RRIDAB_2736835
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24593
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.