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Validation Data Gallery

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  • Western Blot (WB) analysis of U-87 MG cells using IGFBP6 Monoclonal antibody (67567-1-Ig)

    WB analysis of U-87 MG using 67567-1-Ig (same clone as 67567-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 67567-1-Ig (IGFBP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67567-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using IGFBP6 Monoclonal antibody (67567-1-Ig)

    WB analysis of human testis using 67567-1-Ig (same clone as 67567-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 67567-1-Ig (IGFBP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67567-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using IGFBP6 Monoclonal antibody (67567-1-Ig)

    IHC staining of human liver using 67567-1-Ig (same clone as 67567-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67567-1-Ig (IGFBP6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67567-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using IGFBP6 Monoclonal antibody (67567-1-Ig)

    IHC staining of human liver using 67567-1-Ig (same clone as 67567-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67567-1-Ig (IGFBP6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67567-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using IGFBP6 Monoclonal antibody (67567-1-Ig)

    IF Staining of HaCaT using 67567-1-Ig (same clone as 67567-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using IGFBP6 antibody (67567-1-Ig, Clone: 1D12B3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67567-1-PBS in a different storage buffer formulation.

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IGFBP6 Monoclonal antibody, PBS Only

IGFBP6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67567-1-PBS
Clone No.1D12B3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

IGFBP 6, IGF-binding protein 6, IGF binding protein 6, IBP-6, IBP6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67567-1-PBS targets IGFBP6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IGFBP6 fusion protein Ag30050 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor binding protein 6
Calculated molecular weight 25 kDa
Observed molecular weight 30-33 kDa
GenBank accession numberBC011708
Gene Symbol IGFBP6
Gene ID (NCBI) 3489
RRIDAB_2882781
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24592
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Insulin-like growth factor (IGF) binding protein (IGFBP6), a 240 amino acid protein, contains an IGFBP N-terminal domain and a thyroglobulin type-1 domain. It modulates the activity of IGF and shows independent effects of IGF, such as growth inhibition and apoptosis. It can decrease the proliferation and survival of cancer cells such as lung cancer cells and naso-pharyngeal cancer cells. IGFBP-6 is distinctive for its 50-fold higher binding affinity for IGF-II over IGF-I and this specificity makes it an attractive potential therapeutic candidate for IGF-II-dependent pediatric malignancies such as rhabdomyosarcoma (RMS). In addition, it was found that IGFBP6 can promote the migration of RMS cells in an IGF-independent manner, and MAPK pathways were involved in this process. Further study reported that IGFBP6 is one of most highly expressed proteins in varicose vein tissues and is involved in the proliferation of vascular smooth muscle cells (VSMCs), which may provide insights into the underlying pathogenesis of varicose vein.