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Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using IGFBP7 Recombinant antibody (83292-5-RR)

    WB analysis of SKOV-3 using 83292-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83292-5-RR (IGFBP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using IGFBP7 Recombinant antibody (83292-5-RR)

    WB analysis of mouse kidney using 83292-5-RR

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 83292-5-RR (IGFBP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83292-5-RR

    Affinity and Kinetic Characterization of 83292-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83292-5-RR against Human IGFBP7 were performed. The affinity constant is 4.79 nM.

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IGFBP7 Recombinant antibody

IGFBP7 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83292-5-RR
Clone No.240228F11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Insulin-like growth factor-binding protein 7, IGFBP-7, IGF-binding protein 7, IBP-7, IBP7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue, SKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83292-5-RR targets IGFBP7 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor binding protein 7
Calculated molecular weight29 kDa
Observed molecular weight 29-32 kDa
GenBank accession numberNM_001553.2
Gene Symbol IGFBP7
Gene ID (NCBI) 3490
RRIDAB_3670959
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ16270-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IGFBP7 antibody 83292-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols