Validation Data Gallery
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Tested Applications
| Positive WB detected in | human plasma tissue, Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IP | See 4 publications below |
| CoIP | See 1 publications below |
| RIP | See 3 publications below |
Product Information
16402-1-AP targets human IgG heavy chain in WB, IHC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | human IgG heavy chain fusion protein Ag9519 相同性解析による交差性が予測される生物種 |
| Full Name | immunoglobulin heavy constant gamma 4 (G4m marker) |
| Calculated molecular weight | 473 aa, 52 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC025985 |
| Gene Symbol | IGHG4 |
| Gene ID (NCBI) | 3503 |
| RRID | AB_2878254 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01861 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IGHG4 has some fragments with MW 36 kDa (http://www.uniprot.org/uniprot/P01861) and 52 kDa (http://www.ncbi.nlm.nih.gov/protein/CAE45900). This antibody detects all the Heavy chain of human immunoglobulin gamma (IgG), IgG1, IgG2, IgG3, IgG4 included.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for human IgG heavy chain antibody 16402-1-AP | Download protocol |
| IHC protocol for human IgG heavy chain antibody 16402-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Cerebrospinal fluid lipocalin 2 as a novel biomarker for the differential diagnosis of vascular dementia. | ||
Neuropathol Appl Neurobiol Plasma total prion protein as a potential biomarker for neurodegenerative dementia: diagnostic accuracy in the spectrum of prion diseases. | ||
Mol Oncol Secretogranin II impairs tumor growth and angiogenesis by promoting degradation of hypoxia-inducible factor-1α in colorectal cancer. | ||
Cancer Gene Ther miR-451a was selectively sorted into exosomes and promoted the progression of esophageal squamous cell carcinoma through CAB39 | ||
J Dent Sci Circ_0004491 stimulates guanine nucleotide-binding protein alpha subunit to inhibit the malignant progression of oral squamous cell carcinoma by sponging miR-2278 | ||
Oncol Rep Upregulation of IGF1 by tumor-associated macrophages promotes the proliferation and migration of epithelial ovarian cancer cells. |