human IgG heavy chain Polyclonal antibody
human IgG heavy chain Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IP, CoIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 16402-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human plasma tissue, Jurkat cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
IHC | See 2 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
RIP | See 3 publications below |
Product Information
16402-1-AP targets human IgG heavy chain in WB, IHC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | human IgG heavy chain fusion protein Ag9519 相同性解析による交差性が予測される生物種 |
Full Name | immunoglobulin heavy constant gamma 4 (G4m marker) |
Calculated molecular weight | 473 aa, 52 kDa |
Observed molecular weight | 55 kDa |
GenBank accession number | BC025985 |
Gene symbol | IGHG4 |
Gene ID (NCBI) | 3503 |
RRID | AB_2878254 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IGHG4 has some fragments with MW 36 kDa (http://www.uniprot.org/uniprot/P01861) and 52 kDa (http://www.ncbi.nlm.nih.gov/protein/CAE45900). This antibody detects all the Heavy chain of human immunoglobulin gamma (IgG), IgG1, IgG2, IgG3, IgG4 included.
Protocols
Product Specific Protocols | |
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WB protocol for human IgG heavy chain antibody 16402-1-AP | Download protocol |
IHC protocol for human IgG heavy chain antibody 16402-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Cerebrospinal fluid lipocalin 2 as a novel biomarker for the differential diagnosis of vascular dementia. | ||
Neuropathol Appl Neurobiol Plasma total prion protein as a potential biomarker for neurodegenerative dementia: diagnostic accuracy in the spectrum of prion diseases. | ||
Mol Oncol Secretogranin II impairs tumor growth and angiogenesis by promoting degradation of hypoxia-inducible factor-1α in colorectal cancer. | ||
J Dent Sci Circ_0004491 stimulates guanine nucleotide-binding protein alpha subunit to inhibit the malignant progression of oral squamous cell carcinoma by sponging miR-2278 | ||
Oncol Rep Upregulation of IGF1 by tumor-associated macrophages promotes the proliferation and migration of epithelial ovarian cancer cells. | ||
Int J Mol Sci Identification of Potential Biomarkers for Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment by Vitreous iTRAQ-Based Proteomic Profiling. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Pratheepa (Verified Customer) (06-11-2021) | The antibody works excellent. But the specificity is not restricted to human cells. It picks up mouse cell line also.
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