IGJ Polyclonal antibody
IGJ Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13688-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human plasma tissue, mouse spleen tissue, human plasma |
Positive IP detected in | mouse spleen tissue |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
IHC | See 1 publications below |
Product Information
13688-1-AP targets IGJ in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | hamster, human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IGJ fusion protein Ag4559 相同性解析による交差性が予測される生物種 |
Full Name | immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides |
Calculated molecular weight | 158 aa, 18 kDa |
Observed molecular weight | 25 kDa |
GenBank accession number | BC038982 |
Gene symbol | IGJ |
Gene ID (NCBI) | 3512 |
RRID | AB_2877973 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IGJ, the immunoglobulin J chain, serves to link two monomer units of either IgM or IgA.
Protocols
Product Specific Protocols | |
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WB protocol for IGJ antibody 13688-1-AP | Download protocol |
IHC protocol for IGJ antibody 13688-1-AP | Download protocol |
IP protocol for IGJ antibody 13688-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Phytomedicine The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides. | ||
Int Immunopharmacol Integrative analysis-based identification and validation of a prognostic immune cell infiltration-based model for patients with advanced gastric cancer. | ||
J Biotechnol Efficient production of recombinant secretory IgA against Clostridium difficile toxins in CHO-K1 cells. | ||
Plant Mol Biol Plant-derived secretory component gives protease-resistance to Shiga toxin 1-specific dimeric IgA. |