IgG Kappa Light Chain Polyclonal antibody
IgG Kappa Light Chain Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 14678-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | human plasma tissue, human liver tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human spleen tissue, human heart tissue, human placenta tissue, human skin tissue, human testis tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
| ChIP | See 1 publications below |
| RIP | See 6 publications below |
Product Information
14678-1-AP targets IgG Kappa Light Chain in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IgG Kappa Light Chain fusion protein Ag6389 相同性解析による交差性が予測される生物種 |
| Full Name | immunoglobulin kappa locus |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC030813 |
| Gene symbol | IgG Kappa Light Chain |
| Gene ID (NCBI) | 50802 |
| RRID | AB_1851235 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
This antibody detects the light chain (Kappa) of human IgG.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IgG Kappa Light Chain antibody 14678-1-AP | Download protocol |
| IHC protocol for IgG Kappa Light Chain antibody 14678-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice. | ||
PLoS Genet Cytosolic O-GlcNAcylation and PNG1 maintain Drosophila gut homeostasis by regulating proliferation and apoptosis. | ||
J Bone Miner Metab Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1β-induced osteoarthritic chondrocytes. | ||
Mol Med Rep Long non‑coding RNA XIST promotes cerebral ischemia/reperfusion injury by modulating miR‑27a‑3p/FOXO3 signaling. | ||
J Exp Clin Cancer Res RNA N6-methyladenosine reader IGF2BP2 promotes lymphatic metastasis and epithelial-mesenchymal transition of head and neck squamous carcinoma cells via stabilizing slug mRNA in an m6A-dependent manner. | ||
Heliyon HMGA2 overexpression activates IGF2BP2 to stabilize APLP2 via m6A modification and promote pancreatic cancer progression |
