IgG Kappa Light Chain Polyclonal antibody

IgG Kappa Light Chain Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11781-1-AP

Synonyms

IgG Kappa Light Chain, IGKV, IGKV1 5, IGKV15, IGKV1-5, L12, L12a, V1


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Tested Applications

Positive WB detected inhuman plasma tissue, A549 cells, K-562 cells, mouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11781-1-AP targets IgG Kappa Light Chain in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IgG Kappa Light Chain fusion protein Ag2406 相同性解析による交差性が予測される生物種
Full Name immunoglobulin kappa variable 1-5
Calculated molecular weight 239 aa, 26 kDa
Observed molecular weight 26 kDa, 13 kDa
GenBank accession numberBC030814
Gene symbol IgG Kappa Light Chain
Gene ID (NCBI) 28299
RRIDAB_10953529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detects the kappa light chain of human IgG.

Protocols

Product Specific Protocols
WB protocol for IgG Kappa Light Chain antibody 11781-1-APDownload protocol
IHC protocol for IgG Kappa Light Chain antibody 11781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols