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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IgG Kappa Light Chain Polyclonal antibody

IgG Kappa Light Chain Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11781-1-AP

Synonyms

IgG Kappa Light Chain, IGKV, IGKV1 5, IGKV15, IGKV1-5, L12, L12a, V1

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using IgG Kappa Light Chain Polyclonal antibody (11781-1-AP)

    WB analysis of human plasma using 11781-1-AP

    human plasma (0.3ug) were subjected to SDS PAGE followed by western blot with 11781-1-AP (IgG light chain (Kappa) Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using IgG Kappa Light Chain Polyclonal antibody (11781-1-AP)

    WB analysis of A549 using 11781-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 11781-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using IgG Kappa Light Chain Polyclonal antibody (11781-1-AP)

    WB analysis of K-562 using 11781-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11781-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using IgG Kappa Light Chain Polyclonal antibody (11781-1-AP)

    WB analysis of mouse spleen using 11781-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11781-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IgG Kappa Light Chain Polyclonal antibody (11781-1-AP)

    IHC staining of human tonsillitis using 11781-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11781-1-AP (IgG Kappa Light Chain antibody) at dilution of 1:1000 (under 4x lens). heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman plasma tissue, A549 cells, K-562 cells, mouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11781-1-AP targets IgG Kappa Light Chain in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IgG Kappa Light Chain fusion protein Ag2406 相同性解析による交差性が予測される生物種
Full Name immunoglobulin kappa variable 1-5
Calculated molecular weight 239 aa, 26 kDa
Observed molecular weight 26 kDa, 13 kDa
GenBank accession numberBC030814
Gene symbol IgG Kappa Light Chain
Gene ID (NCBI) 28299
RRIDAB_10953529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detects the kappa light chain of human IgG.

Protocols

Product Specific Protocols
WB protocol for IgG Kappa Light Chain antibody 11781-1-APDownload protocol
IHC protocol for IgG Kappa Light Chain antibody 11781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols