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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IgG Lambda Light Chain Polyclonal antibody

IgG Lambda Light Chain Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 11541-1-AP

Synonyms

IGL, immunoglobulin lambda locus, IGL-

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using IgG Lambda Light Chain Polyclonal antibody (11541-1-AP)

    WB analysis of human plasma using 11541-1-AP

    human plasma (0.3 ug) were subjected to SDS PAGE followed by western blot with 11541-1-AP (IgG light chain Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using IgG Lambda Light Chain Polyclonal antibody (11541-1-AP)

    WB analysis of human spleen using 11541-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 11541-1-AP (IgG light chain (lambda) antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IgG Lambda Light Chain Polyclonal antibody (11541-1-AP)

    IHC staining of human tonsillitis using 11541-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11541-1-AP (IgG Lambda Light Chain antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IgG Lambda Light Chain Polyclonal antibody (11541-1-AP)

    IHC staining of human tonsillitis using 11541-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11541-1-AP (IgG Lambda Light Chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IgG Lambda Light Chain Polyclonal antibody (11541-1-AP)

    IF Staining of human tonsillitis using 11541-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IgG Lambda Light Chain antibody (11541-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of Ramos cells using IgG Lambda Light Chain Polyclonal antibody (11541-1-AP)

    FC experiment of Ramos using 11541-1-AP

    1x10^6 Ramos cells were intracellularly stained with 0.4 ug IgG Lambda Light Chain Polyclonal antibody (11541-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inhuman plasma tissue, human spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive FC (Intra) detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11541-1-AP targets IgG Lambda Light Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IgG Lambda Light Chain fusion protein Ag2064 相同性解析による交差性が予測される生物種
Full Name immunoglobulin lambda locus
Calculated molecular weight 237 aa, 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC020233
Gene symbol IgG Lambda Light Chain
Gene ID (NCBI) 3535
RRIDAB_2877775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This antibody detects the lambda light chain of human IgG.

Protocols

Product Specific Protocols
WB protocol for IgG Lambda Light Chain antibody 11541-1-APDownload protocol
IHC protocol for IgG Lambda Light Chain antibody 11541-1-APDownload protocol
IF protocol for IgG Lambda Light Chain antibody 11541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols