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  • Featured Product
  • KD/KO Validated

IGSF11 Polyclonal antibody

IGSF11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14003-1-AP

Synonyms

BT IgSF, BTIGSF, CXADRL1, IGSF11, Igsf13, VSIG3

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using IGSF11 Polyclonal antibody (14003-1-AP)

    WB analysis of human brain using 14003-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14003-1-AP (IGSF11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using IGSF11 Polyclonal antibody (14003-1-AP)

    WB analysis of mouse skeletal muscle using 14003-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14003-1-AP (IGSF11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IGSF11 Polyclonal antibody (14003-1-AP)

    IHC staining of human kidney using 14003-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14003-1-AP (IGSF11 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using IGSF11 Polyclonal antibody (14003-1-AP)

    IHC staining of human kidney using 14003-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14003-1-AP (IGSF11 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inhuman brain tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14003-1-AP targets IGSF11 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGSF11 fusion protein Ag5094 相同性解析による交差性が予測される生物種
Full Name immunoglobulin superfamily, member 11
Calculated molecular weight 430 aa, 46 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC034411
Gene symbol IGSF11
Gene ID (NCBI) 152404
RRIDAB_10603368
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IGSF11 antibody 14003-1-APDownload protocol
IHC protocol for IGSF11 antibody 14003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC,IF

Neurosci Lett

Promotion of differentiation in developing mouse cerebellar granule cells by a cell adhesion molecule BT-IgSF.

Authors - Kasumi Higashine
  • KD Validated
View Article
humanIHC

Medicine (Baltimore)

Prediction of lung squamous cell carcinoma immune microenvironment and immunotherapy efficiency with pyroptosis-derived genes

Authors - Xiaheng Deng
View Article