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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGSF2 Monoclonal antibody (66333-1-Ig)

    WB analysis using 66333-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66333-1-Ig (IGSF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NR8383 cells using IGSF2 Monoclonal antibody (66333-1-Ig)

    WB analysis of NR8383 using 66333-1-Ig

    NR8383 cells were subjected to SDS PAGE followed by western blot with 66333-1-Ig (IGSF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IGSF2 Monoclonal antibody (66333-1-Ig)

    IHC staining of human lung cancer using 66333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IGSF2 Monoclonal antibody (66333-1-Ig)

    IHC staining of human lung cancer using 66333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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IGSF2/CD101 Monoclonal antibody

IGSF2/CD101 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66333-1-Ig
Clone No.1E6A7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

IGSF2, Glu-Trp-Ile EWI motif-containing protein 101, EWI-101, EWI101, EWI 101

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRAW 264.7 cells, NR8383 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66333-1-Ig targets IGSF2/CD101 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IGSF2/CD101 fusion protein Ag23297 相同性解析による交差性が予測される生物種
Full Name immunoglobulin superfamily, member 2
Observed molecular weight 120 kDa
GenBank accession numberBC136484
Gene Symbol IGSF2
Gene ID (NCBI) 9398
RRIDAB_2881713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ93033
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGSF2, also known as CD101, is a low abundance protein. Expression of CD101 is found on monocytes, granulocytes and dendritic cells. In addition expression on T lymphocytes is important for cell activation. It plays a role as inhibitor of T-cells proliferation induced by CD3 and inhibits expression of IL2RA on activated T-cells and secretion of IL2.

Protocols

Product Specific Protocols
WB protocol for IGSF2/CD101 antibody 66333-1-IgDownload protocol
IHC protocol for IGSF2/CD101 antibody 66333-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols