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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IGSF2 Monoclonal antibody

IGSF2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E6A7

Cat no : 66333-1-Ig

Synonyms

CD101, Cell surface glycoprotein V7, EWI 101, EWI101, IGSF2, V7

Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using IGSF2 Monoclonal antibody (66333-1-Ig)

    WB analysis of RAW 264.7 using 66333-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66333-1-Ig (IGSF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IGSF2 Monoclonal antibody (66333-1-Ig)

    IHC staining of human lung cancer using 66333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IGSF2 Monoclonal antibody (66333-1-Ig)

    IHC staining of human lung cancer using 66333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inRAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66333-1-Ig targets IGSF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IGSF2 fusion protein Ag23297 相同性解析による交差性が予測される生物種
Full Name immunoglobulin superfamily, member 2
Observed molecular weight 120 kDa
GenBank accession numberBC136484
Gene symbol IGSF2
Gene ID (NCBI) 9398
RRIDAB_2881713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGSF2, also known as CD101, is a low abundance protein. Expression of CD101 is found on monocytes, granulocytes and dendritic cells. In addition expression on T lymphocytes is important for cell activation. It plays a role as inhibitor of T-cells proliferation induced by CD3 and inhibits expression of IL2RA on activated T-cells and secretion of IL2.

Protocols

Product Specific Protocols
WB protocol for IGSF2 antibody 66333-1-IgDownload protocol
IHC protocol for IGSF2 antibody 66333-1-IgDownload protocol
FC protocol for IGSF2 antibody 66333-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols