IGSF8/CD316 Polyclonal antibody

IGSF8/CD316 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14387-1-AP

Synonyms

CD316, CD81 partner 3, CD81P3, EWI 2, EWI2, IGSF8, KCT 4, KCT4, LIR D1, PGRL


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Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, mouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14387-1-AP targets IGSF8/CD316 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGSF8/CD316 fusion protein Ag5846 相同性解析による交差性が予測される生物種
Full Name immunoglobulin superfamily, member 8
Calculated molecular weight 65 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC053881
Gene symbol IGSF8
Gene ID (NCBI) 93185
RRIDAB_2121881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGSF8 is also known as CD316, PGRL (PG regulatory-like protein), KAI/CD82-associated surface molecule, and CD81-binding partner 3 (CD81P3). IGSF8 contains four immunoglobulin domains, a transmembrane region, and a short cytoplasmic tail that does not bear any signature motif for signal transduction. IGSF8 which is abundantly expressed in olfactory sensory neuron (OSN) axons and their developing synapses. (PMID: 17785435, PMID: 22687584)

Protocols

Product Specific Protocols
WB protocol for IGSF8/CD316 antibody 14387-1-APDownload protocol
IP protocol for IGSF8/CD316 antibody 14387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Ther Nucleic Acids

Exosomal miR-365a-5p derived from HUC-MSCs regulates osteogenesis in GIONFH through the Hippo signaling pathway.

Authors - Ming-Jie Kuang
ratWB

Biochem Biophys Res Commun

Human umbilical cord mesenchymal stem cell-derived exosomes act via the miR-1263/Mob1/Hippo signaling pathway to prevent apoptosis in disuse osteoporosis.

Authors - Bao-Cheng Yang