IHC Prep & Detect Kit for Mouse Primary Antibody
Sample type
FFPE tissue
Assay type
Immunohistochemistry
Secondary antibody host species
Goat
Secondary antibody species specificity
Mouse
Cat no : PK10018
Validation Data Gallery
Product Information
The PK10018 kit is designed for developing a complete IHC workflow on FFPE samples using primary antibodies raised in mice. The kit contains all the basic reagents, starting from antigen retrieval buffer to mounting media, that would be required for IHC staining of tissue sections with mouse primary antibodies. By simply optimizing the primary antibody concentration and a few operational parameters, a complete IHC workflow can be easily established using this kit.
Components
| Part NO. | Component | Size |
| HC001 | Antigen Retrieval Buffer (50× Tris-EDTA) | 100 mL |
| HC002 | Washing Buffer (30×) | 120 mL |
| HC003 | Blocking Buffer | 5 mL |
| HC000 | Quenching Buffer (RTU) | 5 mL |
| HC004 | Primary Antibody Diluent (RTU) | 100 mL |
| HC005M | Polymer HRP-Goat anti-Mouse Secondary Antibody | 5 mL |
| HC006 | Chromogen Component A | 0.2 mL |
| HC007 | Chromogen Component B | 4 mL |
| HC008 | Signal Enhancer | 5 mL |
| HC009 | Counter Staining Reagent | 5 mL |
| HC0010 | Mounting Media | 5 mL |
Size: 50-80 tests (may vary depending on tissue size).
Storage
All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Application Note
This kit is recommended for FFPE tissues and not for frozen sections.
This kit contains an antigen retrieval buffer based on Tris-EDTA by default, which in our experience is suitable for over 90% of targets. However, if a negative result is obtained using this buffer, we recommend trying alternative buffers listed below.
# PR30001 Sodium Citrate Antigen Retrieval Buffer 50x
# PR30014 Protease K Retrieval Buffer (A+B) (Heating is not required with this buffer)
Refer to user's manual for instructions on developing an IHC workflow using this kit.
Documentation
| SDS |
|---|
| IHC Prep & Detect Kit For Mouse Primary Antibody |
Publications
| Application | Title |
|---|---|
Front Oncol Nanocage-incorporated engineered destabilized 3'UTR ARE of ERBB2 inhibits tumor growth and liver and lung metastasis in EGFR T790M osimertinib- and trastuzumab-resistant and ERBB2-expressing NSCLC via the reduction of ERBB2 | |