IHC Prep & Detect Kit for Rabbit/Mouse Primary Antibody
Sample type
FFPE tissue
Assay type
Immunohistochemistry
Secondary antibody host species
Goat
Secondary antibody species specificity
Rabbit/Mouse
Cat no : PK10019
Validation Data Gallery
Product Information
The PK10019 kit is designed for developing a complete IHC workflow on FFPE samples using primary antibodies raised in rabbits/mice. The kit contains all the basic reagents, starting from antigen retrieval buffer to mounting media, that would be required for IHC staining of tissue sections with rabbit/mouse primary antibodies. By simply optimizing the primary antibody concentration and a few operational parameters, a complete IHC workflow can be easily established using this kit.
* If you are planning to use a rabbit primary antibody for staining mouse samples, we recommend using our IHC Prep & Detect Kit for Rabbit Primary Antibody (Cat.NO. PK10017).
Components
| Part NO. | Component | Size |
| HC001 | Antigen Retrieval Buffer (50× Tris-EDTA) | 100 mL |
| HC002 | Washing Buffer (30×) | 120 mL |
| HC003 | Blocking Buffer | 5 mL |
| HC000 | Quenching Buffer (RTU) | 5 mL |
| HC004 | Primary Antibody Diluent (RTU) | 100 mL |
| HC005 | Polymer HRP-Goat anti-Rabbit/Mouse Secondary Antibody | 5 mL |
| HC006 | Chromogen Component A | 0.2 mL |
| HC007 | Chromogen Component B | 4 mL |
| HC008 | Signal Enhancer | 5 mL |
| HC009 | Counter Staining Reagent | 5 mL |
| HC0010 | Mounting Media | 5 mL |
Size: 50-80 tests (may vary depending on tissue size).
Storage
All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Application Note
This kit is recommended for FFPE tissues and not for frozen sections.
This kit contains an antigen retrieval buffer based on Tris-EDTA by default, which in our experience is suitable for over 90% of targets. However, if a negative result is obtained using this buffer, we recommend trying alternative buffers listed below.
# PR30001 Sodium Citrate Antigen Retrieval Buffer 50x
# PR30014 Protease K Retrieval Buffer (A+B) (Heating is not required with this buffer)
Refer to user’s manual for instructions on developing an IHC workflow using this kit.
Documentation
| SDS |
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| IHC Prep & Detect Kit For Rabbit/Mouse Primary Antibody |
Publications
| Application | Title |
|---|---|
Cell Metab Dietary elaidic acid boosts tumoral antigen presentation and cancer immunity via ACSL5 | |
J Ethnopharmacol Combined Epimedii Folium and Ligustri Lucidi Fructus with dexamethasone alleviate the proliferation of airway smooth muscle cells by regulating apoptosis/autophagy | |
Nat Commun Intermittent dietary methionine deprivation facilitates tumoral ferroptosis and synergizes with checkpoint blockade | |
Wound Repair Regen Autophagy ameliorates Pseudomonas aeruginosa-infected diabetic wounds by regulating the toll-like receptor 4/myeloid differentiation factor 88 pathway |