IHCeasy ACTL6A Ready-To-Use IHC Kit
ACTL6A Ready-to-use reagent kit for IHC.
Cat no : KHC0982
Synonyms
actin like 6A, Actin like protein 6A, Actin related protein Baf53a, ACTL6, ACTL6A, Arp4, ARPN BETA, ArpNbeta, BAF53, BAF53A, BRG1 associated factor 53A, INO80 complex subunit K, INO80K
Validation Data Gallery
Product Information
KHC0982 is a ready-to-use IHC kit for staining of ACTL6A. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy ACTL6A Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Actin-like 6A (ACTL6A, synonyms: ACTL6, BAF53A, MGC5382) is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. ACTL6A is a subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | actin like 6A, Actin like protein 6A, Actin related protein Baf53a, ACTL6, ACTL6A, Arp4, ARPN BETA, ArpNbeta, BAF53, BAF53A, BRG1 associated factor 53A, INO80 complex subunit K, INO80K |
