IHCeasy ADAR1 Ready-To-Use IHC Kit
ADAR1 Ready-to-use reagent kit for IHC.
Cat no : KHC0183
Synonyms
ADAR, ADAR1, adenosine deaminase, RNA, DRADA, DSH, DSRAD, G1P1, IFI 4, IFI4, K88dsRBP, p136
Validation Data Gallery
Product Information
KHC0183 is a ready-to-use IHC kit for staining of ADAR1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy ADAR1 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region. The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an IFN inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters. It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH). ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | ADAR, ADAR1, adenosine deaminase, RNA, DRADA, DSH, DSRAD, G1P1, IFI 4, IFI4, K88dsRBP, p136 |
