IHCeasy CPEB3 Ready-To-Use IHC Kit
CPEB3 Ready-to-use reagent kit for IHC.
Cat no : KHC1835
Synonyms
CPE binding protein 3, CPE BP3, CPEB3, hCPEB 3, KIAA0940
Validation Data Gallery
Product Information
KHC1835 is a ready-to-use IHC kit for staining of CPEB3. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy CPEB3 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
CPEB3, the cytoplasmic polyadenylation element-binding protein 3, contains a Q/N-rich domain at its N-termina which is required for the aggregation of CPEB3. The expression of CPEB3 is downregulated in cervical cancer and hepatocellular carcinoma compared with normal tissue. It has been reported that the aggregation and activity of CPEB3 is controlled by SUMOylation. CPEB3 is SUMOylated in hippocampal neurons in the basal state while converted into an active form which is associated with a decrease of SUMOylation and an increase of aggregation. CPEB3 is expressed in cytoplasma in unstimulated neurons but translocates to the nucleus following neuronal stimulation.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | CPE binding protein 3, CPE BP3, CPEB3, hCPEB 3, KIAA0940 |
Publications
| Application | Title |
|---|---|
iScience circKDM1A suppresses bladder cancer progression by sponging miR-889-3p/CPEB3 and stabilizing p53 mRNA |