IHCeasy ESRRA Ready-To-Use IHC Kit
ESRRA Ready-to-use reagent kit for IHC.
Cat no : KHC1525
Synonyms
ERR alpha, ERR1, ERRa, ERRalpha, ESRL1, ESRRA, Estrogen receptor like 1, NR3B1, Steroid hormone receptor ERR1
Validation Data Gallery
Product Information
KHC1525 is a ready-to-use IHC kit for staining of ESRRA. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy ESRRA Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
ERRa, also named as ERR1, ESRL1, and NR3B1, is a 423 amino acid protein, which belongs to the nuclear hormone receptor family. NR3 subfamily. ERRa binds DNA as a monomer or a homodimer. ERRa binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. It can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. he ERR alpha/PGC1alpha complex is a regulator of energy metabolism.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | ERR alpha, ERR1, ERRa, ERRalpha, ESRL1, ESRRA, Estrogen receptor like 1, NR3B1, Steroid hormone receptor ERR1 |