IHCeasy KIF22 Ready-To-Use IHC Kit
KIF22 Ready-to-use reagent kit for IHC.
Cat no : KHC1255
Synonyms
KID, KIF22, kinesin family member 22, Kinesin like protein 4, Kinesin like protein KIF22, KNSL4, OBP, OBP 1, OBP 2
Validation Data Gallery
Product Information
KHC1255 is a ready-to-use IHC kit for staining of KIF22. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy KIF22 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
KIF22, also known as KID (kinesin-like DNA-binding protein), is a kinesin-like DNA-binding protein known to be involved in chromosome movement during mitosis. KIF22 is a nuclear protein regulated by SIAH-1, whose level fluctuate in a cell cycle-dependent manner, increasing during pre-mitotic phases and greatly decreasing during mitosis. KIF22 localizes to the nucleus of the interphase cells upon entry into mitosis and displays some punctate cytoplasmic staining. Defects in KIF22 are the cause of spondyloepimetaphyseal dysplasia with joint laxity, type 2 (SEMDJL2).
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | KID, KIF22, kinesin family member 22, Kinesin like protein 4, Kinesin like protein KIF22, KNSL4, OBP, OBP 1, OBP 2 |
