IHCeasy PRDX5 Ready-To-Use IHC Kit
PRDX5 Ready-to-use reagent kit for IHC.
Cat no : KHC2224
Synonyms
Validation Data Gallery
Product Information
KHC2224 is a ready-to-use IHC kit for staining of PRDX5. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy PRDX5 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Mouse Monoclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Mouse |
| Reactivity | Human |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Peroxiredoxin 5 (PRDX5) is a member of a family consisting of six antioxidant enzymes. PRDX5 is ubiquitously expressed in various tissues including mitochondria and peroxisomes, implying that PRDX5 functions as a regulator of the cellular oxidation state. PRDX5 plays a critical role in protecting cells from oxidative stress by inhibiting the accumulation of reactive oxygen species and cell death. PRDX5 has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. PRDX5 expression was upregulated during adipogenesis and PRDX5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms |