IHCeasy SFRS2 Ready-To-Use IHC Kit
SFRS2 Ready-to-use reagent kit for IHC.
Cat no : KHC0165
Synonyms
PR264, Protein PR264, SC 35, SC35, SFRS2, SFRS2A, Splicing component, 35 kDa, Splicing factor SC35, SRp30b, SRSF2
Validation Data Gallery
Product Information
KHC0165 is a ready-to-use IHC kit for staining of SFRS2. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy SFRS2 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
SFRS2, also named as PR264 and Splicing component, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. Some residue of SFRS2 is modified by phosphorylase and acetylase after translation. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. This kit does not cross react with SFRS2B.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | PR264, Protein PR264, SC 35, SC35, SFRS2, SFRS2A, Splicing component, 35 kDa, Splicing factor SC35, SRp30b, SRSF2 |