IHH Polyclonal antibody
IHH Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13388-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human kidney tissue, HEK-293 cells, human heart tissue |
Positive IHC detected in | human kidney tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IHC | See 5 publications below |
IF | See 3 publications below |
Product Information
13388-1-AP targets IHH in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IHH fusion protein Ag4046 相同性解析による交差性が予測される生物種 |
Full Name | Indian hedgehog homolog (Drosophila) |
Calculated molecular weight | 411 aa, 45 kDa |
Observed molecular weight | 45 kDa |
GenBank accession number | BC034757 |
Gene symbol | IHH |
Gene ID (NCBI) | 3549 |
RRID | AB_2248725 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for IHH antibody 13388-1-AP | Download protocol |
IHC protocol for IHH antibody 13388-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. | ||
Cell Mol Life Sci Adamts17 is involved in skeletogenesis through modulation of BMP-Smad1/5/8 pathway. | ||
Mol Ther Intra-articular Delivery of Antago-miR-483-5p Inhibits Osteoarthritis by Modulating Matrilin 3 and Tissue Inhibitor of Metalloproteinase 2. | ||
Osteoarthritis Cartilage mTORC1 activation downregulates FGFR3 and PTH/PTHrP receptor in articular chondrocytes to initiate osteoarthritis. | ||
Int J Mol Sci Inhibition of Ihh Reverses Temporomandibular Joint Osteoarthritis via a PTH1R Signaling Dependent Mechanism. | ||
Dev Biol DDB1 promotes the proliferation and hypertrophy of chondrocytes during mouse skeleton development. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Hongxue (Verified Customer) (05-13-2022) | WB works well.
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