Validation Data Gallery
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Tested Applications
| Positive WB detected in | human kidney tissue, HEK-293 cells, human heart tissue |
| Positive IHC detected in | human kidney tissue, human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 5 publications below |
| IF | See 4 publications below |
Product Information
13388-1-AP targets IHH in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IHH fusion protein Ag4046 相同性解析による交差性が予測される生物種 |
| Full Name | Indian hedgehog homolog (Drosophila) |
| Calculated molecular weight | 411 aa, 45 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC034757 |
| Gene Symbol | IHH |
| Gene ID (NCBI) | 3549 |
| RRID | AB_2248725 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14623 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IHH antibody 13388-1-AP | Download protocol |
| IHC protocol for IHH antibody 13388-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. | ||
Cell Mol Life Sci Adamts17 is involved in skeletogenesis through modulation of BMP-Smad1/5/8 pathway. | ||
Mol Ther Intra-articular Delivery of Antago-miR-483-5p Inhibits Osteoarthritis by Modulating Matrilin 3 and Tissue Inhibitor of Metalloproteinase 2. | ||
Osteoarthritis Cartilage mTORC1 activation downregulates FGFR3 and PTH/PTHrP receptor in articular chondrocytes to initiate osteoarthritis. | ||
Int J Mol Sci Inhibition of Ihh Reverses Temporomandibular Joint Osteoarthritis via a PTH1R Signaling Dependent Mechanism. | ||
Dev Biol DDB1 promotes the proliferation and hypertrophy of chondrocytes during mouse skeleton development. |