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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IK Polyclonal antibody (31833-1-AP)

    WB analysis using 31833-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31833-1-AP (IK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IK Polyclonal antibody (31833-1-AP)

    IHC staining of mouse testis using 31833-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31833-1-AP (IK antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using IK Polyclonal antibody (31833-1-AP)

    IF Staining of A431 using 31833-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using IK antibody (31833-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

IK Polyclonal antibody

IK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31833-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RED, Protein RER, Protein Red, IK factor, IK Cytokine

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31833-1-AP targets IK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IK fusion protein Ag37095 相同性解析による交差性が予測される生物種
Full Name IK cytokine, down-regulator of HLA II
Calculated molecular weight 557 aa, 66 kDa
Observed molecular weight70 kDa
GenBank accession numberBC013005
Gene Symbol IK
Gene ID (NCBI) 3550
RRIDAB_3670123
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ13123
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IK, also known as the nuclear protein RED, is a down-regulator of human leukocyte antigen (HLA) II and interacts with the human SAC protein MAD1, which is required for the localization of the kinetochore of MAD1, for the progression of mitosis and for the activation of the SAC. IK is therefore an important protein for the correct progression of mitosis through the regulation of mitotic kinases and phosphatases. In addition, IK may be involved in pre-mRNA splicing, which may be a useful biomarker for new nuclear structures in cells.

Protocols

Product Specific Protocols
WB protocol for IK antibody 31833-1-APDownload protocol
IHC protocol for IK antibody 31833-1-APDownload protocol
IF protocol for IK antibody 31833-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols