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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IKBKG Monoclonal antibody, PBS Only

IKBKG Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2E2

Cat no : 66460-1-PBS

Synonyms

AMCBX1, FIP 3, FIP3, Fip3p, I kappa B kinase subunit gamma, IkB kinase subunit gamma, IKBKG, IKK gamma, IKKAP1, IKKG, IP, IP1, IP2, IPD2, NEMO, NF kappa B essential modifier, NF kappa B essential modulator

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IKBKG Monoclonal antibody (66460-1-Ig)

    WB analysis using 66460-1-Ig (same clone as 66460-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66460-1-Ig (IKBKG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IKBKG Monoclonal antibody (66460-1-Ig)

    IHC staining of human breast cancer using 66460-1-Ig (same clone as 66460-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66460-1-Ig (IKBKG antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IKBKG Monoclonal antibody (66460-1-Ig)

    IHC staining of human breast cancer using 66460-1-Ig (same clone as 66460-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66460-1-Ig (IKBKG antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66460-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66460-1-PBS targets IKBKG in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IKBKG fusion protein Ag13358 相同性解析による交差性が予測される生物種
Full Name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC012114
Gene symbol IKBKG
Gene ID (NCBI) 8517
RRIDAB_2881829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways.