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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-1 Beta Polyclonal antibody (16806-1-AP)

    WB analysis using 16806-1-AP (same clone as 16806-1-PBS)

    Non-treated THP-1 cells, THP-1 cells (treated with 100 ng/ml LPS for 3h, then added 300 ng/ml protein transport inhibitor for 3h) were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL-1 Beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using IL-1 Beta Polyclonal antibody (16806-1-AP)

    WB analysis of A431 using 16806-1-AP (same clone as 16806-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL1 beta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human adrenal gland tissue using IL-1 Beta Polyclonal antibody (16806-1-AP)

    WB analysis of human adrenal gland using 16806-1-AP (same clone as 16806-1-PBS)

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL1 beta antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-1 Beta Polyclonal antibody (16806-1-AP)

    IHC staining of human tonsillitis using 16806-1-AP (same clone as 16806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-1 Beta Polyclonal antibody (16806-1-AP)

    IHC staining of human tonsillitis using 16806-1-AP (same clone as 16806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IL-1 Beta Polyclonal antibody (16806-1-AP)

    IHC staining of human kidney using 16806-1-AP (same clone as 16806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-1 Beta Polyclonal antibody (16806-1-AP)

    IHC staining of human tonsillitis using 16806-1-AP (same clone as 16806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-1 Beta Polyclonal antibody (16806-1-AP)

    IHC staining of human tonsillitis using 16806-1-AP (same clone as 16806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-1 Beta Polyclonal antibody (16806-1-AP)

    IHC staining of human tonsillitis using 16806-1-AP (same clone as 16806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-1 Beta Polyclonal antibody (16806-1-AP)

    IHC staining of human tonsillitis using 16806-1-AP (same clone as 16806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IL-1 Beta Polyclonal antibody (16806-1-AP)

    IHC staining of human kidney using 16806-1-AP (same clone as 16806-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16806-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

IL-1 Beta Polyclonal antibody, PBS Only

IL-1 Beta Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16806-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16806-1-PBS targets IL-1 Beta in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-1 Beta fusion protein Ag10295 相同性解析による交差性が予測される生物種
Full Name interleukin 1, beta
Calculated molecular weight 269 aa, 31 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC008678
Gene Symbol IL1B
Gene ID (NCBI) 3553
ENSEMBL Gene IDENSG00000125538
RRIDAB_10646432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01584
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.