IL-1 beta Recombinant antibody
IL-1 beta Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
240902D6
Cat no : 82696-15-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LPS and Brefeldin A treated THP-1 cells, A431 cells, HT-1080 cells, U-87 MG cells |
Positive IF/ICC detected in | LPS and Brefeldin A treated THP-1 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 82696-15-RR is IL-1 beta Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Fusion Protein 相同性解析による交差性が予測される生物種 |
Full Name | interleukin 1, beta |
Calculated molecular weight | 269 aa, 31 kDa |
Observed molecular weight | 30-35 kDa |
GenBank accession number | BC008678 |
Gene symbol | IL1B |
Gene ID (NCBI) | 3553 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.
Protocols
Product Specific Protocols | |
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WB protocol for IL-1 beta antibody 82696-15-RR | Download protocol |
IF protocol for IL-1 beta antibody 82696-15-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |