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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-1 alpha Recombinant antibody, PBS Only

IL-1 alpha Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241752F1

Cat no : 84411-5-PBS

Synonyms

IL 1 alpha, IL1A, IL-1,IL1, IL1 ALPHA, IL 1A

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using IL-1 alpha Recombinant antibody (84411-5-RR)

    WB analysis of THP-1 using 84411-5-RR (same clone as 84411-5-PBS)

    PMA and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 84411-5-RR (IL-1 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84411-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IL-1 alpha Recombinant antibody (84411-5-RR)

    IHC staining of human colon cancer using 84411-5-RR (same clone as 84411-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84411-5-RR (IL-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84411-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IL-1 alpha Recombinant antibody (84411-5-RR)

    IHC staining of human colon cancer using 84411-5-RR (same clone as 84411-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84411-5-RR (IL-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84411-5-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84411-5-PBS is IL-1 alpha Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IL-1 alpha fusion protein HZ-1320 相同性解析による交差性が予測される生物種
Full Name interleukin 1, alpha
Calculated molecular weight 271 aa, 31 kDa
Observed molecular weight31 kDa
GenBank accession numberBC013142
Gene symbol IL-1 alpha
Gene ID (NCBI) 3552
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin-1α (IL-1 alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.