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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-1 beta Polyclonal antibody (26048-1-AP)

    WB analysis using 26048-1-AP (same clone as 26048-1-PBS)

    LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26048-1-AP (IL-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IL-1 beta Polyclonal antibody (26048-1-AP)

    WB analysis using 26048-1-AP (same clone as 26048-1-PBS)

    LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 26048-1-AP (IL-1 beta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IL-1 beta Polyclonal antibody (26048-1-AP)

    WB analysis using 26048-1-AP (same clone as 26048-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26048-1-AP (IL-1 beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26048-1-PBS in a different storage buffer formulation.

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Mouse IL-1 beta Polyclonal antibody, PBS Only

IL-1 beta Polyclonal Antibody for WB, Indirect ELISA
Cat No. 26048-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

IL 1beta, Il1b, IL-1,IL1, IL-1 beta,IL 1 beta,IL1beta, Il 1b

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26048-1-PBS targets IL-1 beta in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-1 beta fusion protein Ag23461 相同性解析による交差性が予測される生物種
Full Name interleukin 1 beta
Calculated molecular weight 31 kDa
Observed molecular weight31 kDa
GenBank accession numberNM_008361
Gene Symbol IL-1 beta
Gene ID (NCBI) 16176
RRIDAB_2880351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.