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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-1 beta Recombinant antibody

IL-1 beta Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240902G6

Cat no : 82696-17-RR

Synonyms

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-1 beta Recombinant antibody (82696-17-RR)

    WB analysis using 82696-17-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82696-17-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using IL-1 beta Recombinant antibody (82696-17-RR)

    WB analysis of THP-1 using 82696-17-RR

    LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82696-17-RR (IL-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82696-17-RR

    Affinity and Kinetic Characterization of 82696-17-RR

    Biolayer interferometry (BLl) kinetic assays of 82696-17-RR against Human IL-1 beta were performed. The affinity constant is 60.2 nM.

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Tested Applications

Positive WB detected inA431 cells, LPS and Brefeldin A treated THP-1 cells, HT-1080 cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82696-17-RR is IL-1 beta Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name interleukin 1, beta
Calculated molecular weight 269 aa, 31 kDa
Observed molecular weight35 kDa
GenBank accession numberBC008678
Gene symbol IL1B
Gene ID (NCBI) 3553
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to the activation of numerous downstream targets.

Protocols

Product Specific Protocols
WB protocol for IL-1 beta antibody 82696-17-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols