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Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using IL-1 beta Recombinant antibody (84136-5-RR)

    WB analysis of THP-1 using 84136-5-RR

    LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 84136-5-RR (IL-1 beta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84136-5-RR

    Affinity and Kinetic Characterization of 84136-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84136-5-RR against Mouse IL-1 beta were performed. The affinity constant is 13.2 pM.

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IL-1 beta Recombinant antibody

IL-1 beta Recombinant Antibody for WB, ELISA
Cat No. 84136-5-RR
Clone No.241364D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

IL 1beta, Il1b, IL-1,IL1, IL-1 beta,IL 1 beta,IL1beta, Il 1b

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inLPS and Brefeldin A treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84136-5-RR targets IL-1 beta in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 1 beta
Calculated molecular weight31kDa
Observed molecular weight31 kDa
GenBank accession numberNM_008361.4
Gene Symbol IL-1 beta
Gene ID (NCBI) 16176
RRIDAB_3671695
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10749
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.

Protocols

Product Specific Protocols
WB protocol for IL-1 beta antibody 84136-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols