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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IL-1 beta Rabbit Recombinant Antibody (98142-1-RR)

    FC experiment of human PBMCs using 98142-1-RR

    1x10^6 untreated or IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-1 beta Rabbit Recombinant Antibody (98142-1-RR, Clone: 240902A3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Granulocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IL-1 beta Rabbit Recombinant Antibody (98142-1-RR)

    FC experiment of human PBMCs using 98142-1-RR

    1x10^6 IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-1 beta Rabbit Recombinant Antibody (98142-1-RR, Clone: 240902A3) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 unstimulated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-1 beta Rabbit Recombinant Antibody (98142-1-RR, Clone: 240902A3) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Granulocytes were gated.

  • Affinity and Kinetic Characterization of 98142-1-RR

    Affinity and Kinetic Characterization of 98142-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98142-1-RR against Human IL-1 beta were performed. The affinity constant is 4.98 nM.

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Anti-Human IL-1 beta Rabbit Recombinant Antibody

IL-1 beta Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98142-1-RR
Clone No.240902A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC (Intra) detected inIFN gamma, LPS and Brefeldin A treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98142-1-RR targets IL-1 beta in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name interleukin 1, beta
Calculated molecular weight 269 aa, 31 kDa
GenBank accession numberBC008678
Gene Symbol IL1B
Gene ID (NCBI) 3553
ENSEMBL Gene IDENSG00000125538
RRIDAB_3672288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01584
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.

Protocols

Product Specific Protocols
FC protocol for IL-1 beta antibody 98142-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols